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Fig. 4 | BMC Plant Biology

Fig. 4

From: Identification and functional analysis of the geranylgeranyl pyrophosphate synthase gene (crtE) and phytoene synthase gene (crtB) for carotenoid biosynthesis in Euglena gracilis

Fig. 4

Analysis of phytoene production in E. coli by HPLC. HPLC chromatogram (284 nm) of extracts from E. coli carrying a pET-EgcrtE with pAC-PacrtB, b pACCRT-E [23] with pET-EgcrtB and c pET-EgcrtEB. d Absorbance spectrum of phytoene detected at a retention time of 28.6 min. Phytoene was extracted from E. coli transformants and analyzed with HPLC in accordance with the method of Takaichi [57]. The arrowheads in the chromatograms indicate the position of phytoene elution. Data are representative of three or four experiments with similar results

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BMC Plant Biology

ISSN: 1471-2229

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