Fig. 4

Analysis of phytoene production in E. coli by HPLC. HPLC chromatogram (284 nm) of extracts from E. coli carrying a pET-EgcrtE with pAC-PacrtB, b pACCRT-E [23] with pET-EgcrtB and c pET-EgcrtEB. d Absorbance spectrum of phytoene detected at a retention time of 28.6 min. Phytoene was extracted from E. coli transformants and analyzed with HPLC in accordance with the method of Takaichi [57]. The arrowheads in the chromatograms indicate the position of phytoene elution. Data are representative of three or four experiments with similar results