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Fig. 6 | BMC Plant Biology

Fig. 6

From: The loss-of-function GLABROUS 3 mutation in cucumber is due to LTR-retrotransposon insertion in a class IV HD-ZIP transcription factor gene CsGL3 that is epistatic over CsGL1

Fig. 6

Relative transcript abundances of CsGL1 and CsGL3 genes in different genetic backgrounds (WT, csgl3, csgl1 and csgl1 + 3). a Expression of CsGL3 is nearly undetectable in csgl3 (a, b) and csgl1 + 3 double mutant (a), and it is reduced by approximately half in csgl1 mutant. b Semi RT-PCR of CsGL3 between RIL-46 M and RIL-46 W suggests the transcript of 4th exon is missing in the mutant. c PCR cloning of the full-length cDNA of CsGL3 between RIL-46 M and RIL-46 W suggests missing of the 4th exon sequence in the transcript of the mutant line. d Expression of CsGL1 was almost undetectable in all mutant lines

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