Table 1 PCR primers details
Primers | Primer sequence (5'-3') | Primer design | Annealing temp. °C | Amplified region | Allelic variant | PCR product size (bp) |
|---|---|---|---|---|---|---|
VRN1AF | gaaaggaaaaattctgctcg | Yan et al. [19] | 60 | VRN-A1 promoter | vrn-A1 | 713 |
VRN1-INT1R | gcaggaaatcgaaatcgaag | Vrn-A1a.1 | 853, 944 | |||
Vrn-A1a.2 | 853, 924, 944 | |||||
Vrn-A1a.3 | 765,853 | |||||
vrn-A m 1 | 705 | |||||
Vrn-A1b | 691 | |||||
Vrn-A1d | 685 | |||||
Vrn-A m 1g | 681 | |||||
Vrn-A m 1a | 671 | |||||
Vrn-A1e | 659 | |||||
Vrn-A1f | 658 | |||||
vrn-A m 1b | 656 | |||||
Vrn-A1-intr_F | ccgtcgaaaggatcgctactg | This study | 60 | VRN-A1 intron-1 | vrn-A1 | 541 |
Vrn-A1-intr_R1 | cttgtccccgtgagctacttac | 60 | ||||
Ex1/C/F | gttctccaccgagtcatggt | Fu et al. [20] | 58 | VRN-A1 intron-1 | Vrn-A1c (Langdon) | 522 |
Intr1/A/R3 | aagtaagacaacacgaatgtgaga | 58 | Vrn-A1c (IL369) | 2188 | ||
Pr1 | tacccctgctaccagtgcct | Shcherban et al. [22] | 60 | VRN-B1 promoter | VRN-B1.f | 968 |
Pr2 | ggccaaccctacaccccaag | 60 | VRN-B1.s | 958 | ||
VRN-B1.m | 965 | |||||
Pr1 | tacccctgctaccagtgcct | Shcherban et al. [22] | 60 | VRN-B1 promoter | Vrn-B1(ins) | 1256 |
PrB/Ins/r2 | tgcggtacgggttatccag | This study | 60 | |||
Ex1/C/F | gttctccaccgagtcatggt | Fu et al. [20] | 61 | VRN-B1 intron-1 | Vrn-B1a | 1091 |
Intr1/B/R3 | ctcatgccaaaaattgaagatga | 61 | Vrn-B1b | 1055 | ||
Vrn-B1c | 705 | |||||
Ex1/C/F | gttctccaccgagtcatggt | Fu et al. [20] | 61 | VRN-B1 intron-1 | vrn-B1 | 1531 |
Intr1/B/R4 | caaatgaaaaggaatgagagca | 61 | ||||
FT-B-INS-F | cataatgccaagccggtgagtac | Yan et al. [3] | 63 | VRN-B3 promoter | Vrn-B3a | 1765 |
VRN4-B-NOINS-R | ctatccctaccggccattag | 63 | ||||
VRN4-B-NOINS-F2 | gctgtgtgatcttgctctcc | Yan et al. [3] | 63 | VRN-B3 promoter | vrn-B3 | 691 |
VRN4-B-NOINS-R | ctatccctaccggccattag | 63 | vrn-B3b | 1581 |