Fig. 3

Effect of CLE-peptides on the development of Raphanus raphanistrum and Raphanus sativus roots. a-h—Effect of exogenous CLE peptides supplying. Transverse sections of Raphanus sativus line 19 (a-d) and Raphanus raphanistrum (e-h) roots of 15-day old plants after 7 days cultivation of medium with synthetic CLE-peptides: control (CLE41def) (a, e), CLE19p (b, f), CLE41p (c, g), and CLE2p (d, h). i-m Effect of RsCLE41 overexpression on development of meristematic foci in the central part of xylem zone. i, j transverse sections of mature (30 day old) roots of Raphanus sativus line 19: i—GUS overexpression (control); j RsCLE41 overexpression. k, l—meristematic foci in the central part of xylem zone of roots with GUS overexpression (k) and RsCLE41 overexpression (l). m number of xylem elements on transverse section of Raphanus sativus root of 250 μM in diameter (P < 0.01 - **, P < 0.001 - ***). n qRT-PCR analysis of RsCLE41 expression in GUS- overexpressing and RsCLE41-overexpressing Raphanus sativus roots (lines 19 and 27)