Fig. 5

Relative amounts of patatin, PMC and Ser protease inhibitors (PI’s) in tuber flesh of control line K and corn cystatin II (CCII)-expressing lines 9.4 and 10.4 stored for 0 or 30 weeks at 4 °C. a SDS-PAGE protein profile of total soluble proteins in tubers of line K after storage for 0 or 30 weeks. b Residual amounts of patatin, PMC or Ser PI’s after 30 weeks compared to initial amounts at time 0 (arbitrary value of 1.00). Similar amounts of tuber crude extract were loaded in each well. Each bar on panel (b) is the mean of three biological (tuber replicate) values ± sd. For each protein, bars with a different letter are significantly different (post-anova Tukey’s test, P < 0.05). See ref. [26] for MS/MS identification of the tuber test proteins following electrophoresis