Fig. 10

Transient expression of ClMPK7 triggered HR-like cell death and accumulation of H₂O₂. Agrobacteria harboring constructs containing ClMPK7 or eGFP (a negative control) were infiltrated into leaves of 4-week-old N. benthamiana plants. a Detection of ClMPK7 in ClMPK7-transiently expressed leaves. Leaf samples were harvested at 24 h after agroinfiltration and total soluble proteins were extracted. Proteins were separated by SDS–PAGE and analyzed by immunoblotting using a GFP-specific antibody. Total proteins showing equal loading were examined by Coomassie staining. b HR-like cell death in ClMPK7-transiently expressed leaves. Photos were taken at 7 days after agroinfiltration and representative leaves showing HR-like cell death (large necrotic lesions) were particularly presented. c Accumulation of H₂O₂. Leaf samples were collected at 24 h after agroinfiltration and H₂O₂ accumulation was detected by DAB staining. Repeated experiments showed similar results