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Table 2 aSelected differentially expressed trichome-related leaf ESTs detected by RNA sequencing of hairy AtGL3+ B. napus and ultra-hairy K-5-8 relative to semi-glabrous Westar

From: Hairy Canola (Brasssica napus) re-visited: Down-regulating TTG1 in an AtGL3-enhanced hairy leaf background improves growth, leaf trichome coverage, and metabolite gene expression diversity

Gene ID Arabidopsis homologue bFPKM Hairy line (Sample 1) FPKM Westar (Sample 2) clog2 (FC-S1/S2) P_value
Hairy AtGL3+ B. napus relative to semi-glabrous Westar
Bra016164 AT1G71030,ATMYBL2,MYBL2, MYB like 2 91.65 7.27 3.66 5.63E-04
Bo7g096780 AT5G24520,TTG1,TTG,URM23,ATTTG1, Transducin WD40 repeat like superfamily protein 7.26 0.58 3.64 2.95E-03
Bo6g014160 AT1G71030,ATMYBL2,MYBL2, MYB like 2 32.04 2.91 3.46 6.66E-04
Bo6g046840 AT1G79840,GL2, HD ZIP IV family of homeo, leucine zipper protein with lipid binding START domain 7.61 0.86 3.14 9.69E-03
Bo3g025000 AT2G31110, Plant protein of unknown function DUF828 2.20 19.66 −3.16 1.97E-03
Bra016578 AT1G17920,HDG12, homeodomain GLABROUS 12 0.38 6.02 −4.00 2.94E-03
Ultra-hairy K-5-8 relative to semi-glabrous Westar
Bra003535 AT1G79840,GL2, HD ZIP IV family of homeo, leucine zipper protein with lipid binding START domain 1.60 0.31 2.38 6.03E-03
Bra016164 AT1G71030,ATMYBL2,MYBL2, MYB like 2 88.18 7.27 3.60 0.00E+00
Bo6g014160 AT1G71030,ATMYBL2,MYBL2, MYB like 2 30.33 2.91 3.38 3.95E-10
Bo6g046840 AT1G79840,GL2, HD ZIP IV family of homeo, leucine zipper protein with lipid binding START domain 3.37 0.86 1.96 4.15E-03
Bo2g070770 AT1G71030,ATMYBL2,MYBL2, MYB like 2 10.86 2.86 1.93 3.70E-04
Bo9g083130 AT5G52510,SCL8, SCARECROW like 8 9.11 2.41 1.92 1.45E-09
Bra028312 AT5G52510,SCL8, SCARECROW like 8 5.34 1.61 1.73 9.64E-05
Bo8g080200 AT3G50800, unknown protein 13.53 4.47 1.60 4.35E-03
Bra036862 AT3G50800, unknown protein 24.99 11.63 1.10 2.16E-03
Bra024875 AT2G01570,RGA1,RGA, GRAS family transcription factor family protein 33.57 20.95 0.68 1.11E-03
Bo3g013120 AT5G18410,PIR,KLK,PIR121,SRA1,PIRP,ATSRA1, transcription activators 6.06 9.65 −0.67 1.01E-02
Bo4g039020 AT2G35110,GRL,NAP1,NAPP, transcription activators 9.66 15.68 −0.70 2.44E-03
Bo1g078380 AT3G48750,CDKA;1,CDC2AAT,CDK2,CDC2,CDC2A,CDKA1, cell division control 2 12.99 22.38 −0.78 1.01E-02
Bra018866 AT1G50660, unknown protein 3.55 6.17 −0.80 2.53E-03
Bo6g010070 AT1G73360,HDG11,EDT1,ATHDG11, homeodomain GLABROUS 11 6.82 11.97 −0.81 3.04E-03
Bra009218 AT5G06700,TBR, Plant protein of unknown function DUF828 4.02 7.27 −0.86 7.21E-04
Bo8g077280 AT3G48750,CDKA;1,CDC2AAT,CDK2,CDC2,CDC2A,CDKA1, cell division control 2 8.26 15.62 −0.92 6.49E-04
Bra036731 AT1G33240,AT GTL1,AT GTL2,GTL1, GT 2 like 1 AT1G33240,AT GTL1,AT GTL2,GTL1, GT 2 like 1 AT1G33240,AT GTL1,AT GTL2,GTL1, GT 2 like 1 AT1G33240,AT GTL1,AT GTL2,GTL1, GT 2 like 1 5.78 10.96 −0.92 1.90E-03
Bo2g096060 AT1G80350,ERH3,AAA1,FRA2,LUE1,ATKTN1,KTN1,FRC2, FTR, P loop containing nucleoside triphosphate hydrolases superfamily protein 9.78 19.03 −0.96 8.26E-03
Bra018036 AT3G48750,CDKA;1,CDC2AAT,CDK2,CDC2,CDC2A,CDKA1, cell division control 2 6.23 12.17 −0.97 7.66E-04
Bo8g027460, Bo8g027470 AT1G33240,AT GTL1,AT GTL2,GTL1, GT 2 like 1 11.99 23.41 −0.97 2.44E-03
Bo6g077400 AT1G33240,AT GTL1,AT GTL2,GTL1, GT 2 like 1 AT1G33240,AT GTL1,AT GTL2,GTL1, GT 2 like 1 AT1G33240,AT GTL1,AT GTL2,GTL1, GT 2 like 1 AT1G33240,AT GTL1,AT GTL2,GTL1, GT 2 like 1 20.53 40.86 −0.99 3.56E-05
Bo7g040300 AT3G23590,RFR1,MED33A, REF4 related 1 5.03 10.02 −0.99 2.13E-04
Bra040010 AT1G33240,AT GTL1,AT GTL2,GTL1, GT 2 like 1 AT1G33240,AT GTL1,AT GTL2,GTL1, GT 2 like 1 AT1G33240,AT GTL1,AT GTL2,GTL1, GT 2 like 1 AT1G33240,AT GTL1,AT GTL2,GTL1, GT 2 like 1 9.77 21.52 −1.14 1.30E-05
Bo6g049350 AT3G61150,HDG1,HD GL2 1, homeodomain GLABROUS 1 3.11 7.35 −1.24 4.85E-07
Bra003439 AT3G61150,HDG1,HD GL2 1, homeodomain GLABROUS 1 2.67 6.62 −1.31 8.62E-06
Bo7g116660 AT4G34160,CYCD3;1,CYCD3, CYCLIN D3 1.86 4.68 −1.33 2.01E-03
Bra005177 AT2G37630,ATPHAN,AS1,ATMYB91,MYB91, myb like HTH transcriptional regulator family protein 5.35 13.47 −1.33 1.04E-08
Bo5g021100 AT1G15570,CYCA2;3, CYCLIN A2 6.64 17.09 −1.36 7.19E-06
Bo5g136400 AT3G11540,SPY, Tetratricopeptide repeat TPR like superfamily protein 8.20 21.31 −1.38 2.98E-08
Bra035000 AT3G44200,ATNEK6,NEK6,NIMA never in mitosis, gene A related 6 1.09 2.85 −1.39 1.68E-05
Bra026120 AT1G15570,CYCA2;3, CYCLIN A2 1.96 5.24 −1.42 1.13E-05
Bo8g039430 AT4G14970, unknown protein 0.91 2.52 −1.47 2.17E-03
Bo3g001470 AT5G01360,TBL3, Plant protein of unknown function DUF828 0.69 2.39 −1.79 2.15E-03
Bo7g093130 AT5G28640,AN3,GIF,GIF1,ATGIF1, SSXT family protein 1.55 5.52 −1.83 3.19E-03
Bo1g007590 AT4G34160,CYCD3;1,CYCD3, CYCLIN D3 2.71 10.20 −1.91 1.34E-08
Bra011501 AT4G34160,CYCD3;1,CYCD3, CYCLIN D3 1.27 4.78 −1.92 6.07E-06
Bo8g067530 AT1G17920,HDG12, homeodomain GLABROUS 12 1.26 5.93 −2.24 3.38E-11
Bra022827 AT2G31110, Plant protein of unknown function DUF828 0.73 3.66 −2.32 4.54E-04
Bo3g024610 AT2G30432,TCL1, Homeodomain like superfamily protein 8.99 49.82 −2.47 6.37E-05
Bra016578 AT1G17920,HDG12, homeodomain GLABROUS 12 1.09 6.02 −2.47 1.07E-06
Bra000011 AT2G37630,ATPHAN,AS1,ATMYB91,MYB91, myb like HTH transcriptional regulator family protein 2.77 17.22 −2.64 1.41E-09
Bo3g025000 AT2G31110, Plant protein of unknown function DUF828 1.82 19.66 −3.43 0.00E+00
  1. aGenes were selected by screening RNA sequencing data for 50 common trichome genes described in [61]. Expression changes in 65 less common differentially expressed trichome genes are also found in Additional file 2: Table S2A (statistically different expression from Westar) and Additional file 2: Table S2B (not statistically different). b FPKM Fragment Per Kilobase of exon per Million fragments mapped. clog2 FC-S1/S2, log2 of the fold change (hairy leaf sample 1/semi-glabrous leaf sample 2). P_value indicates significant differences of the means of a hairy line relative to the semi-glabrous Westar line