Hairy Canola (Brasssica napus) re-visited: Down-regulating TTG1 in an AtGL3-enhanced hairy leaf background improves growth, leaf trichome coverage, and metabolite gene expression diversity

Table 1 Transformation efficiency with BnTTG1 manipulation: Survival and viable seed set of confirmed transformants

Genetic background	Binary construct	No. of explants	Putative T₀ PPT-tolerant plantlets^a	Confirmed T₀transgenic plants^a	Single insertion locus plants^a	T₀ plants with viable seed set
B. napus Westar	O-TTG1	500	20	12	5	10
B. napus Westar	K-TTG1	500	20	7	2	5
AtGL3⁺ B. napus	O-TTG1	500	9	7	2	1
AtGL3⁺ B. napus	K-TTG1	500	10	9	4	5

^aTransgene presence was confirmed by initial screening using phosphinothricin selection, followed by PCR (Additional file 1: Figure S2) and Southern blotting (Additional file 1: Figure S3) of the selectable marker gene (BAR) to confirm transgene loci number

ISSN: 1471-2229