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Fig. 5 | BMC Plant Biology

Fig. 5

From: Hairy Canola (Brasssica napus) re-visited: Down-regulating TTG1 in an AtGL3-enhanced hairy leaf background improves growth, leaf trichome coverage, and metabolite gene expression diversity

Fig. 5

Combined relative expression (representing all orthologues and paralogues) of six trichome regulatory genes by Q-PCR in two leaf tissue batches of seedlings from semi-glabrous B. napus cv Westar (W), hairy AtGL3+ B. napus, ultra-hairy K-5-8 (BnTTG1 knock-down in AtGL3+ B. napus), and glabrous T3 O-3-7 (BnTTG1 over-expressed in AtGL3+ B. napus). Batch 1: 1st to 3rd true leaves; Batch 2: 4th to 6th true leaves. Relative expression levels for AtGL3, BnTTG1, BnGL1, BnGL2, BnGL3, and BnTRY are plotted relative to the expression levels of the same genes in glabrous cotyledons of the B. napus cv Westar control line (set at 1; data not shown). The latter (cotyledon) expression was also normalized to the B. napus cotyledon expression of the ACETYL TRANSFERASE 2 (ACT2), which was found to be stable across cotyledon and leaf growth stages (relative to the stable EF1 house-keeping gene). A Tukey test (for each gene) compared the mean relative expression between the four different plant lines and different leaf batches. One cDNA sample per tissue batch per plant was plated into three wells (technical replicates) from each of three plants (biological reps) per line. Cotyledons of five Westar plants (technical reps) were combined to make one control biological replicate. Significantly different means of three individual plants per line (± SE) are indicated by different letters (P ≤ 0.05)

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