Table 2 Description of genes implicated in auxin, cytokinin, abscisic acid, brassinolide biosynthesis and primers used for qRT-PCR
Gene | Molecular function | Gene ID in carrotDB | Primer sequences (forward/reverse) |
|---|---|---|---|
DcYUCCA | Indole-3-pyruvate monooxygenase | comp48938 | GTTCTGTCCAGTCCGAGGTTGAG/CCTCTCCTCCGAACTCTTGTAATCC |
DcCYP83B1 | Cytochrome P450 83B1 | comp43558 | GACATGCTGAGTACGGCAGTTACC/TCCAATGAATGGAAGTCCAGGAGGA |
DcIPT3 | Adenylate isopentenyltransferase | comp34617 | GAATGGAATGGTAGATGAGGCAAGACA/TCTCTAACTGGCGGCAGGCTAG |
DcABA2 | Xanthoxin dehydrogenase | comp50471 | CGATATTATGGTCAACAATGCGGGTAT/CGTCGTGCTGCTTACACTACTCA |
DcMoCo | MoCo sulfurase | comp15442 | CCTGGAACTGATTGGAATACCGAAGT/GCCTGGATTAATGGAATAGACGCCTTA |
DcOPR2 | OPDA reductase | comp45214 | CACATGGTGGAGCCAAGGATGAA/AGCAACAAGGTCAGTACAGTTCTCAG |
DcDAD1 | Lipase | comp46390 | CTCGAAGAGGAGCTTAGTGAAGTTGTT/TTAGGAAGAAGAAGGACTCGGCAATAC |
DcDWF4 | Cytochrome P450 90B1 | comp42688 | AAACGCTAAGGCTGGGCAATGT/GCACGGCTGCAATCACTGGAA |
DcDWF5 | Sterol delta-7 reductase | comp15451 | AGATGGTGGTGAAGGAGGAGAA/CACGCAGTCATAGTGGGTTTTG |