Table 2 qRT-PCR validations of 12 randomly selected genes from the differentially expressed genes in the RNA-seq results
Gene ID | Gene annotation | Ratio in RNA-seq (before inoculation) | Fold-change in qRT-PCR (before inoculation) | Fold-change in qRT-PCR (24Â h after inoculation) |
|---|---|---|---|---|
LOC_Os01g03940 | Negative regulator of disease resistance , expressed protein | 4.02 | 4.36 | 5.39 |
LOC_Os01g12160 | OsGH3.3-Probable indole-3-acetic acid-amido synthetase, expressed | 3.90 | 3.06 | 4.64 |
LOC_Os01g36070 | nodulin MtN3 family protein, putative, expressed | 4.93 | 3.30 | 1.23 |
LOC_Os02g39660 | receptor kinase, putative, expressed | 0.003 | 0.09 | 0.25 |
LOC_Os02g40784 | WAX2, putative, expressed | 0.50 | 0.45 | 0.28 |
LOC_Os05g25770 | Superfamily of TFs having WRKY and zinc finger domains, expressed | 15.34 | 10.94 | 3.6 |
LOC_Os06g38830 | receptor-like protein kinase precursor, putative, expressed | 0.11 | 0.25 | 0.73 |
LOC_Os09g25070 | OsWRKY62 - Superfamily of TFs having WRKY and zinc finger domains, expressed | 22.75 | 8.89 | 6.43 |
LOC_Os11g12050 | NBS-LRR type disease resistance protein, putative, expressed | 0.17 | 0.37 | 0.62 |
LOC_Os11g12320 | disease resistance protein RPM1, putative | 0.026 | 0.35 | 0.81 |
LOC_Os11g31190 | nodulin MtN3 family protein, putative, expressed | 2.78 | 6.48 | 8.01 |
LOC_Os12g36880 | pathogenesis-related Bet v I family protein, putative, expressed | 3.13 | 2.14 | 0.77 |