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Table 5 Quantification of total sugars from WT and galt mucilage sequentially extracted using ammonium oxalate, 0.2 N NaOH, and 2 N NaOH

From: A small multigene hydroxyproline-O-galactosyltransferase family functions in arabinogalactan-protein glycosylation, growth and development in Arabidopsis

 

Extracta

Genotype

Ammonium oxalate

0.2 N NaOH

2 N NaOH

WT

0.85 ± 0.05

1.04 ± 0.03

0.81 ± 0.04

galt1-2

0.83 ± 0.07

1.05 ± 0.05

0.84 ± 0.03

galt2-1

0.95 ± 0.03b

1.12 ± 0.04b

0.61 ± 0.05b

galt2-2

0.97 ± 0.70b

1.19 ± 0.03b

0.54 ± 0.06b

galt3-1

1.30 ± 0.09c

1.29 ± 0.05c

0.53 ± 0.05b

galt3-2

1.28 ± 0.05c

1.27 ± 0.07c

0.52 ± 0.07b

galt4-1

0.88 ± 0.20

1.09 ± 0.02

0.79 ± 0.01

galt4-2

0.90 ± 0.60b

1.01 ± 0.06

0.73 ± 0.03

galt5-1

0.95 ± 0.10b

1.17 ± 0.05b

0.63 ± 0.05b

galt5-2

0.90 ± 0.08b

1.20 ± 0.07c

0.60 ± 0.04b

galt6-1

1.25 ± 0.09c

1.30 ± 0.07c

0.55 ± 0.05b

galt6-2

1.31 ± 0.05c

1.29 ± 0.04c

0.61 ± 0.08b

galt2galt5

1.40 ± 0.09d

1.36 ± 0.08d

0.47 ± 0.05d

  1. aIntact seeds were extracted sequentially with 0.2 % ammonium oxalate, 0.2 N NaOH and 2 N NaOH, neutralized, and assayed by the phenol-sulfuric acid method against glucose standards. The results are shown as μg/mg of seeds. Analyses were performed in triplicate and results are given as μg/mg seed ± SE. All genotypes were grown, harvested, and stored together. Letters ‘b’ ‘c’ and ‘d’ denote a significantly difference from the wild type (Dunnett’s test, P <0.05; P <0.01; P <0.001 respectively)