Fig. 5

Schematic gene models, locations of T-DNA mutant insertions, and transcript analysis of GALT1, GALT3, GALT4, and GALT6. a GALT1, GALT3. GALT4 and GALT6 gene structures and T-DNA insertion sites in galt1-1, galt1-2, galt3-1, galt3-2, galt4-1, galt4-2, galt6-1, and galt6-2 mutants. The intron-exon structures of GALT1, GALT3, GALT4, and GALT6 are indicated (introns are drawn as lines and exons as rectangles, with white rectangles representing coding sequences and black rectangles representing UTRs). Sites of T-DNA insertions are marked (triangles) as are the locations of primer sequences (arrows above the genes) used for PCR screening. b RT-PCR analysis of transcripts from rosette leaves of 14-day-old WT (Col-0) and allelic homozygous galt1, galt3, galt4 and galt6 mutant lines. Arrows below the genes in (a) indicate the position of primers (denoted as RTF and RTR) used for RT-PCR analysis of transcript levels. UBQ10 primers were used as internal controls. c Quantitative real-time reverse transcription -PCR (qRT-PCR) analysis was performed to quantify and compare transcript levels of the indicated genes with that of corresponding WT gene. In other words, the relative expression level of the GALT genes in the mutants was compared to WT values, which were set to a value of 1.0 for each of the GALT genes. Asterisks indicate values significantly different from the WT expression of the indicated genes (Dunnett’s test, *P <0.01; **P <0.001)