Fig. 4From: A small multigene hydroxyproline-O-galactosyltransferase family functions in arabinogalactan-protein glycosylation, growth and development in ArabidopsisSubcellular localization of transiently expressed GALT3-YFP, GALT4-YFP, and GALT6-YFP in N. tabacum. GALT3-YFP, GALT4-YFP, and GALT6-YFP fusion constructions were expressed under the control of the CaMV 35S promoter in N. tabacum. Transiently expressed GALT3-YFP, GALT4-YFP, and GALT6-YFP co-localized with sialyl transferase (ST)-GFP fusion protein (a Golgi marker), but not with HDEL-GFP fusion protein (an ER marker). These constructs were examined by laser-scanning confocal microscopy under fluorescent and white light, and the fluorescent images were merged to observe co-localization. Size bar = 10 μmBack to article page