Skip to main content
Fig. 6 | BMC Plant Biology

Fig. 6

From: The effect of UV-B on Arabidopsis leaves depends on light conditions after treatment

Fig. 6

Influence of 5 min UV-B (8 W·m−2) irradiation on the senescence and cell death of Arabidopsis leaves. After irradiation samples were kept either in darkness or under constant light (100 μmol·m−2·s−1) for given time. a-d Time-course of the relative expression of senescence associated genes: (a) SAG13, (b) SAG12, (c) SEN1 and (d) WRKY53 normalized for the expression of four housekeeping genes (PDF2, UBC9, UBQ10, SAND). Day 0 means 1 h after the treatment. Non-irradiated leaf halves served as a control. After the specified time period leaves were cut into halves and control and treated halves from 4 different leaves were pooled. Each measurement was repeated at least 3 times. A single dark-adapted overnight control sample from day 0 was used as a reference for calculating relative expression levels. Error bars indicate the standard error. e Trypan blue staining for cell death of leaves irradiated with UV-B with the middle part covered and transferred either to light or to dark conditions

Back to article page