Table 2 Representative Arabidopsis RING proteins used in this study, their expression in a wheat germ cell-free system and their ubiquitination activities
AGI loci | Other names | RING type | M. wt. (Da) | High molecular smear | Activity with UBC8/10 | Activity with other UBCs | Comments |
|---|---|---|---|---|---|---|---|
At1g22500 | ATL15 | H2 | 42,226 | No(a) | Yes(c) | UBC11, UBC28 and UBC29(e) | |
At1g49200 | H2 | 24,790 | Yes(b) | nd | nd | ||
At3g09760 | v | 23,097 | Yes(a) | Yes(c) | UBC11(e) | ||
At2g39100 | HCa | 31,376 | No(a) | Yes(d) | nd | ||
At1g74410 | H2 | 12,293 | No(a) | No(c), Yes(d) | UBC8(f) | ||
At5g07270 | XBAT33 | HCa | 56,010 | Yes(a) | Yes(c) | UBC11, UBC28(e) | |
At1g73760 | H2 | 40,616 | No(a) | Yes(d) | nd | ||
At1g80400 | H2 | 44,551 | Yes(a) | nd | nd | ||
At1g71980 | H2 | 46,218 | Yes(a) | nd | nd | ||
At1g15100 | RHA2a | H2 | 16,983 | No(a) | Yes(c) | UBC11, UBC28 and UBC29(e) | |
At5g08139 | H2 | 60,426 | Yes(b) | nd | nd | ||
At2g18670 | H2 | 21,067 | No(a) | No(c) | nd | Low expression | |
At3g47550 | v | 31,784 | Yes(b) | nd | nd | ||
At2g47700 | RFI2 | H2 | 35,729 | No(a) | Yes(c) | UBC11, UBC28 and UBC29(e) | |
At1g74760 | H2 | 29,318 | No(a) | No(c) | nd | ||
At3g23280 | XBAT35 | HCa | 50,055 | No(a) | Yes(c) | UBC11, UBC28(e) | |
At2g04240 | XERICO | H2 | 17,928 | Yes(a) | nd | nd | |
At5g22920 | RZPF34 | H2 | 33,549 | No(a) | No(c) | nd | |
At3g05200 | ATL6 | H2 | 42,561 | No(a) | Yes(c) | UBC11, UBC28 and UBC29(e) | Low expression |
At4g39140 | H2 | 47,548 | No(a) | No(c) | nd | ||
At2g32950 | COP1/DET340/EMB168/FUS1 | HCa | 76,188 | Yes(a) | nd | nd | |
At2g44950 | HUB1/RDO4 | HCa | 59,491 | No(a) | Yes(d) | UBC1, UBC2 and UBC8(f) | |
At5g45290 | H2 | 60,928 | No(a) | Yes(d) | nd | ||
At5g01520 | AIRP2 | HCa | 28,050 | No(a) | No(c) | nd | |
At2g15580 | H2 | 23,182 | No(a) | No(c), Yes(d) | UBC8, UBC31(f) | ||
At5g03180 | v | 52,475 | Yes(a) | nd | nd | ||
At1g21650 | SECA2 | 177,644 | Yes(a) | nd | nd | Truncated | |
At1g79810 | PEX2/TED3 | HCa | 38,175 | Yes(a) | nd | nd | |
At2g35330 | HCa | 79,194 | Yes(a) | Yes(d) | nd | ||
At1g63170 | H2 | 42,677 | Yes(a) | nd | nd | ||
At3g09770 | AIRP3/LOG2 | HCa | 42,848 | No(a) | Yes(c) | UBC11, UBC28 and UBC29(e) | |
At4g25230 | RIN2 | H2 | 66,537 | Yes(a) | nd | nd | Low expression |
At1g12760 | H2 | 40,453 | Yes(a) | Yes(c) | UBC11, UBC28 and UBC29(e) | Low expression | |
At5g06420 | HCa | 42,460 | No(a) | Yes(d) | nd | ||
At5g15820 | H2 | 38,560 | Yes(b) | nd | nd | ||
At4g23450 | AIRP1 | H2 | 21,143 | No(a) | Yes(c) | UBC11, UBC28 and UBC29(e) | |
At2g02960 | v | 29,591 | Yes(b) | nd | nd | ||
At1g55530 | H2 | 38,963 | Yes(b) | nd | nd | ||
At2g28840 | XBAT31 | HCa | 46,391 | No(a) | No(c) | nd | |
At3g47160 | HCa | 28,524 | No(a) | Yes(c) | UBC11(e) | ||
At5g20910 | AIP2 | H2 | 34,807 | No(a) | Yes(c) | UBC11, UBC28, UBC29 and UBC30(e) | |
At5g14420 | RGLG2 | HCa | 51,578 | No(a) | Yes(c) | UBC11, UBC28, UBC29 and UBC30(e) | |
At4g11680 | H2 | 47,218 | Yes(a) | Yes(c) | UBC11, UBC28, UBC29, UBC35 and UBC36(e) | ||
At5g51450 | RIN3 | H2 | 65,087 | Yes(a) | nd | nd | Low expression |
At3g61550 | H2 | 23,224 | No(a) | Yes(d) | nd | ||
At3g06330 | v | 46,711 | No(a) | Yes(c) | UBC11, UBC28 and UBC29(e) | Low expression | |
At3g16720 | ATL2 | H2 | 34,052 | No(a) | No(c), Yes(d) | UBC8, UBC31(f) | |
At5g18260 | H2 | 35,673 | No(a) | Yes(d) | nd | ||
At5g01960 | HCa | 46,018 | Yes(a) | nd | nd | ||
At5g62460 | v | 33,660 | Yes(b) | nd | nd | ||
At1g69330 | HCa | 30,423 | No(a) | Yes(d) | nd | ||
At2g35910 | H2 | 19,725 | No(a) | Yes(d) | nd | ||
At2g22680 | WAVH1 | H2 | 74,401 | No(a) | Yes(c) | UBC11, UBC28 and UBC29(e) | |
At5g63780 | SHA1 | H2 | 39,838 | Yes(a) | nd | nd | |
At5g63760 | ARI15 | HCb | 57,610 | Yes(a) | nd | nd | |
At3g61180 | H2 | 40,754 | Yes(a) | nd | nd | ||
At1g74370 | HCa | 29,400 | Yes(a) | Yes(c) | UBC11, UBC28, UBC29, UBC35 and UBC36(e) | ||
At5g37930 | HCa | 38,924 | Yes(a) | nd | nd | ||
At2g22120 | v | 28,253 | Yes(a) | nd | nd | ||
At1g18470 | HCa | 47,408 | Yes(a) | nd | nd | ||
At4g19670 | HCb | 60,343 | No(a) | Yes(d) | nd | ||
At4g09560 | H2 | 48,030 | Yes(a) | nd | nd | ||
At5g15790 | H2 | 26,352 | No(a) | Yes(d) | nd | ||
At1g60610 | HCa | 38,334 | No(a) | No(c), Yes(d) | nd | ||
At3g16090 | HRD1a | H2 | 56,037 | Yes(a) | nd | nd | |
At1g11020 | v | 31,326 | Yes(a) | nd | nd | ||
At1g50440 | H2 | 28,785 | Yes(a) | Yes(c) | nd | ||
At4g14220 | RHF1a | H2 | 41,035 | Yes(a) | Yes(c) | nd | |
At4g35840 | H2 | 29,169 | Yes(a) | nd | nd | ||
At3g07200 | HCa | 20,090 | Yes(b) | nd | nd | ||
At1g23980 | H2 | 40,765 | No(a) | Yes(d) | nd | ||
At4g33940 | HCa | 73,413 | No(a) | Yes(d) | nd | Truncated | |
At2g38920 | HCa | 35,490 | Yes(a) | nd | nd | ||
At1g74870 | C2 | 33,404 | No(a) | Yes(d) | nd | ||
At3g48070 | C2 | 35,234 | No(a) | Yes(c) | UBC11(e) | ||
At1g30860 | HCa | 84,259 | Yes(a) | nd | nd |