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Table 1 Summary of Arabidopsis E2s used in this study, their protein expression in a wheat germ cell-free system and activity as E2 enzymes

From: Wheat germ-based protein libraries for the functional characterisation of the Arabidopsis E2 ubiquitin conjugating enzymes and the RING-type E3 ubiquitin ligase enzymes

Gene name

AGI loci

Other names

Subfamily

M. wt. (Da)

Protein expression

Thioester formation

UBC1

At1g14400(a)

 

III

17,280

Yes

Yes

UBC2

At2g02760(a)

 

III

17,270

Yes

Yes

UBC3

At5g62540(a)

 

III

17,130

Yes

Yes

UBC4

At5g41340(a)

 

IV

21,300

Yes

Yes

UBC5

At1g63800(a)

 

IV

19,400

Yes

Yes

UBC6

At2g46030(a)

 

IV

20,890

Yes

Yes

UBC7

At5g59300(b)

 

V

22,900

Yes

Yes

UBC13

At3g46460(a)

 

V

18,820

Yes

Yes

UBC14

At3g55380(a)

 

V

18,720

Yes

Yes

UBC8

At5g41700(a)

 

VI

16,530

Yes

Yes

UBC9

At4g27960(a)

 

VI

16,550

Yes

Yes

UBC10

At5g53300(a)

 

VI

16,530

Yes

Yes

UBC11

At3g08690(a)

 

VI

16,550

Yes

Yes

UBC12

At3g08700(c)

 

VI

16,710

Yes

Yes

UBC28

At1g64230(a)

 

VI

19,270

Yes

Yes

UBC29

At2g16740(a)

 

VI

16,760

Yes

Yes

UBC30

At5g56150(a)

 

VI

16,480

Yes

Yes

UBC15

At1g45050(a)

ATUBC2-1

VII

18,260

Yes

Yes

UBC16

At1g75440(a)

 

VII

18,490

Yes

Yes

UBC17

At4g36410(b)

 

VII

18,670

Yes

Yes

UBC18

At5g42990(a)

 

VII

18,370

Yes

Yes

UBC19

At3g20060(a)

 

VIII

20,000

Yes

Yes

UBC20

At1g50490(a)

 

VIII

21,460

Yes

Yes

UBC21

At5g25760(b)

PEX4

IX

17,710

No

nd

UBC22

At5g05080(a)

 

X

27,400

Yes

Yes

UBC23

At2g16920(b)

PFU2

XI

122,190

Yes

Yes

UBC24

At2g33770(a)

PHO2

XI

100,490

Yes

Yes

UBC25

At3g15355(a)

PFU1

XI

67,780

Yes

Yes

UBC26

At1g53025(a)

PFU3

XI

60,554

Yes

Yes

UBC27

At5g50870(a)

 

XII

21,250

Yes

Yes

UBC31

At1g36340(b)

 

XIII

17,830

Yes

Yes

UBC32

At3g17000(a)

 

XIV

34,320

Yes

Yes

UBC33

At5g50430(a)

 

XIV

27,360

Yes

Yes

UBC34

At1g17280(a)

 

XIV

26,610

Yes

Yes

UBC35

At1g78870(b)

UBC13A

XV

17,190

Yes

Yes

UBC36

At1g16890(a)

UBC13B

XV

17,220

Yes

Yes

UBC37

At3g24515(c)

 

XVI

45,080

No

nd

  1. The UBC names and subfamilies used here are based on the nomenclature and classification of Arabidopsis E2s described previously [13]. ‘M. wt. (Da)’ indicates the expected molecular weight of the expressed proteins according to the RAFL database or TAIR v10. Abbreviations: yes, detected; No, not detected; nd, proteins not assayed for activity. ‘Thioester formation’ indicates whether DTT-sensitive Ub adducts for E2s were observed (Fig. 2). acDNA from RAFL. bORF was amplified from a commercially available Arabidopsis cDNA library (Stratagene). cORF was amplified by nested PCR from cDNA of 2-week-old plants treated with 100 μM ABA