Skip to main content
Fig. 6 | BMC Plant Biology

Fig. 6

From: Wheat germ-based protein libraries for the functional characterisation of the Arabidopsis E2 ubiquitin conjugating enzymes and the RING-type E3 ubiquitin ligase enzymes

Fig. 6

Wheat germ-based in vitro E2-E3 specificity screening. The activity of four FLAG-tagged RING E3s was tested in the presence of members of different subgroups of N-bio-E2s and HA-Ub. Arabidopsis E2s used in each assay are indicated above each lane according to their UBC number and minus (–) refers to the absence of E2; this condition was used as a negative control. The E2–E3 activity was visualized after SDS-PAGE by anti-HA-HRP immunoblotting. The number below each lane represents the signal intensity quantified by imageJ as normalized to the lane lacking for E2 (–)

Back to article page