Skip to main content
Fig. 5 | BMC Plant Biology

Fig. 5

From: Wheat germ-based protein libraries for the functional characterisation of the Arabidopsis E2 ubiquitin conjugating enzymes and the RING-type E3 ubiquitin ligase enzymes

Fig. 5

Wheat germ-based in vitro ubiquitination assays of various types of RING proteins. 23 FLAG-tagged RING proteins of various RING types were mixed with FLAG-Ub in the presence or absence of N-bio-UBC10 as indicated by plus (+) or minus (–) above each lane. E3 activity was determined by the presence or absence of a smear when FLAG-Ub was detected by anti-FLAG-HRP antibody. This is indicated by plus (+) or minus (–), respectively, below each lane respectively

Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us