Table 1 Primers of the reference and target genes used in qRT-PCR analysis, with GenBank accession/locus numbers
From: Biochar-amended potting medium reduces the susceptibility of rice to root-knot nematode infections
Genes | GenBank accession or locus number | Primer sequences (5’ → 3’) | Function |
|---|---|---|---|
OsEif5C | SM00515 | F: CACGTTACGGTGACACCTTTT | Reference gene |
R: GACGCTCTCCTTCTTCCTCAG | |||
OsEXP | LOC_Os03g27010 | F: TGTGAGCAGCTTCTCGTTTG | Reference gene |
R: TGTTGTTGCCTGTGAGATCG | |||
OsEXPnarsai | LOC_Os07g02340 | F: AGGAACATGGAGAAGAACAAGG | Reference gene |
R: CAGAGGTGGTGCAGATGAAA | |||
OsGSL1 | AP001389 | F: TGAGGACCTGCCACGATT | Callose biosynthesis |
R: CACGCTGATTGCGAACAT | |||
OsRbohB | NM001049555.1 | F: CTGGACAGGACCAAGAGCAG | H2O2 production |
R: ATCTTGAACGGAGCAGCACA | |||
OsEBP89 | LOC_Os03g08460 | F: TGACGATCTTGCTGAACTGAA | ET response |
R: CAATCCCACAAACTTTACACA | |||
OsERF70 | AF193803.1 | F: ACCTTGGGGGTAGCATATCG | ET response |
R: AGGGAACAGGTCCAATCACC | |||
OsERF1 | LOC_Os04g46220 | F: GAGTCGTCCTTCTCCTCCTC | ET response |
R: CCTCTCTTTCTCCGTTTCG | |||
OsACS1 | AK071011 | F: GATGGTCTCGGATGATCACA | ET biosynthesis |
R: GTCGGGGGAAAACTGAAAAT | |||
OsACO7 | LOC_Os01g39860 | F: GGACTACTACCAGGGCACCA | ET biosynthesis |
R: GATTAGCGCACGCGATTTTA | |||
OsEIN2 | LOC_Os07g06130 | F: TAGGGGGACTTTGACCATTG | ET signaling |
R: TGGAAGGGACCAGAAGTGTT |