Fig. 8

Spatial expression pattern of MtD27 upon phosphate starvation. a Transgenic M. truncatula root expressing pMtD27::GUS grown in full nutrient condition (200 μM PO₄ ^3-). b Longitudinal section of the root shown in (a). c Expression of pMtD27::GUS in M. truncatula transgenic roots after 5 days of phosphate starvation (0 μM PO₄ ^3-). d Longitudinal section of the root shown in (c). e Relative transcript abundance as determined by qRT-PCR of MtD27 in wild type (WT) and the Mtdmi3 mutant and Mtnsp1 Mtnsp2 double mutant after 2 days of phosphate starvation (0 μM PO₄ ^3-). f Relative transcript abundance as determined by qRT-PCR of MtD27 in WT and the Mtern1 mutant and Mtnsp1 Mtnsp2 double mutant after 2 days of phosphate starvation. Scale bars are equal to 250 μm in (a) and (c) and 50 μm in (b) and (d). Sections were counterstained with Ruthenium Red. Data in (e,f) represent means of 3 biological replicates that each were analyzed in 3-fold (technical replicates) ± SEM. For each gene, transcript abundance was normalized against that of the mock-treated wild type. Different letters above bars indicate statistical difference (p < 0.05, students’ t-test)