Fig. 4
From: The strigolactone biosynthesis gene DWARF27 is co-opted in rhizobium symbiosis
Symbiotic MtD27 expression is under direct control of S. meliloti LCO signaling. a Relative transcript abundance as determined by qRT-PCR of MtD27 and MtENOD11 in M. truncatula root susceptible zones after 0, 1, 2 or 3 h of LCO treatment (10-9 M). b Relative transcript abundance as determined by qRT-PCR of MtD27 at 3 h after mock (-LCO) or rhizobium LCO (10-9 M) (+LCO) treatment in wild type, Mtdmi1, Mtdmi2 and Mtdmi3. c Relative transcript abundance as determined by qRT-PCR of MtD27 at 3 h after mock (-LCO) or rhizobium LCO (10-9 M) (+LCO) treatment in wild type, Mtnsp1, Mtnsp2 and Mtnsp1 Mtnsp2. d Relative transcript abundance as determined by qRT-PCR of MtENOD11 at 3 h after mock (-LCO) or rhizobium LCO (10-9 M) (+LCO) treatment in wild type and Mtern1. e Relative transcript abundance as determined by qRT-PCR of MtD27 at 3 h after mock (-LCO) or rhizobium LCO (10-9 M) (+LCO) treatment in wild type and Mtern1. f Relative transcript abundance as determined by qRT-PCR of MtD27 and MtENOD11 after mock (-LCO) or rhizobium LCO (10-9 M) (+LCO) treatment, in presence or absence of 50 μM cycloheximide (CHX). Data shown represent means of 3 biological replicates that each were analyzed in 3-fold (technical replicates) ± SEM. For each gene, transcript abundance was normalized against that of the mock-treated wild type. Different letters above bars indicate statistical difference (p < 0.05, students’ t-test)