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Fig. 2 | BMC Plant Biology

Fig. 2

From: The strigolactone biosynthesis gene DWARF27 is co-opted in rhizobium symbiosis

Fig. 2

Effect of MtD27 knock-down on strigolactone biosynthesis. a Relative transcript abundance as determined by qRT-PCR of MtD27 and Medtr7g095920 in M. truncatula transgenic roots expressing an empty vector control construct (EVi) or MtD27 RNAi construct (D27i). Relative transcript abundance was normalized against MtD27 transcript abundance in roots transformed with the empty vector control (EVi). b Relative quantity of the strigolactone didehydro-orobanchol (DDH) (peak area/g FW) in root exudates collected from M. truncatula transgenic roots expressing an empty vector control construct (EVi) or MtD27 RNAi construct (D27i). c Relative quantity of the strigolactone didehydro-orobanchol (DDH) (peak area/g FW) in root extracts collected from M. truncatula transgenic roots expressing an empty vector control construct (EVi) or MtD27 RNAi construct (D27i). Data shown represent means of 4-5 biological replicates ± SEM. Different letters above bars indicate statistical difference (p < 0.05, students’ t-test)

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