Fig. 4

Quantitative RT-PCR validations on microarray data of Cd-responsive transporter genes in barley leaves. a The values represent the gene expression in 5 μM Cd over those in the control in Zhenong8 (grey bars) and W6nk2 (white bars) (n = 3 biological replicates). ACT2 was used as the reference gene. Data plotted are the mean ratio of gene expression in Cd treatment (5 μM Cd for 15 days) over those in the control in the two genotypes on a log₂ scale (b). GDAS, Glutamine-dependent asparagine synthetise; PALT, Phosphoribosylanthranilate transferase; IGPS, Indole-3-glycerol phosphate synthase; HSP, Heat shock protein