Skip to main content

Table 1 Relative product formation of KS(L) enzymes

From: One amino acid makes the difference: the formation of ent-kaurene and 16α-hydroxy-ent-kaurane by diterpene synthases in poplar

 

Ent-isokaurene (%)

Ent- kaurene (%)

Hydroxy-ent-kaurane (%)

PtTPS19

0

100

0

PtTPS20(T→M)

0

100

0

PtTPS20

5.8 ± 1.7

8.1 ± 0.2

86.1 ± 1.5

PtTPS19(M→T)

5.8 ± 3.2

5.9 ± 1.1

88.3 ± 3.9

PtTPS19(M→A)

10.8 ± 2.5

6.9 ± 0.9

82.2 ± 3.4

  1. The enzymes were expressed in E. coli, extracted, partially purified, and incubated with PtTPS17 and the substrate GGPP. Products were extracted with hexane and analyzed by GC-MS Means (n = 3) and standard errors (SE) are shown
Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us