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Fig. 8 | BMC Plant Biology

Fig. 8

From: One amino acid makes the difference: the formation of ent-kaurene and 16α-hydroxy-ent-kaurane by diterpene synthases in poplar

Fig. 8

Putative reaction mechanism for ent-kaurene and 16α-hydroxy-ent-kaurane formation in poplar. The class II terpene synthase PtTPS17 catalyzes the conversion of GGPP into ent-CPP. Two highly similar class I enzymes, PtTPS19 and PtTPS20, accept ent-CPP as a substrate and convert it into ent-kaur-16-ene, the precursor for gibberellin biosynthesis or 16α-hydroxy-ent-kaurane, respectively. The product specificity seems to be controlled by one amino acid in the protein active center excluding (1) or allowing (2) the quenching of the beyeran-16-yl cation by a water molecule (modified from [33]). 3, ent-kaurene; 4, ent-isokaurene; 5, 16α-hydroxy-ent-kaurane

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