Fig. 3From: One amino acid makes the difference: the formation of ent-kaurene and 16α-hydroxy-ent-kaurane by diterpene synthases in poplarGC-MS analysis of diterpenoids produced by recombinant PtTPS17, PtTPS18, PtTPS19 and PtTPS20. The enzymes were expressed in E. coli, extracted, partially purified, and incubated with the substrate GGPP. Products were extracted with hexane and analyzed by GC-MS. 1, geranyllinalool; 2, copalol; 3, ent-kaurene; 4, ent-isokaurene; 5, 16α-hydroxy-ent-kauraneBack to article page