Fig. 6
A model for carbon flux diversion towards fructan biosynthesis in perennial ryegrass. The model is based on the unigenes related to KEGG pathways. Enzymes involved in fructan metabolism: sucrose-sucrose 1-fructosyltransferase (1-SST), fructan-fructan 1-fructosyltransferase (1-FFT), fructan-fructan 6G-fructosyltransferase (6G-FFT), sucrose-fructan 6-fructosyltransferase (6-SFT), and fructan exohydrolases (1-FEH and 6-FEH). KEGG enzyme data base entry numbers (EC) of other enzymes involved in carbon flux diversion towards fructan biosynthesis: glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12), GAPDH (NADP+) (EC 1.2.1.13), fructose-bisphosphate aldolase (EC 4.1.2.13), fructose-bisphosphatase (EC 3.1.3.11), fructose-6-phosphate,2-kinase (EC 2.7.1.105), fructose-2,6-bisphosphatase (EC 3.1.3.46), sucrose synthase (EC 2.4.1.13), UDP-glucose pyrophosphorylase (EC 2.7.7.9) and glucose phosphomutase (EC 5.4.2.2). Metabolic intermediates: glyceraldehyde-3-phosphate (Glyceraldehyde-3P), β-D-frutose 1,6-bisphosphate (β-D-frutose 1,6P2), β-D-fructose 6-phosphate (β-D-fructose 6P), and β-D-fructose 2,6-bisphosphate (β-D-fructose 2,6P2). The expression patterns of the transcripts from perennial ryegrass variety ‘Veyo’ (blue) and ecotype ‘Falster’ (red) showing deduced amino acid sequence similarity with KEGG enzymes are shown. Expression profiles are only shown in cases where significant differential expression was identified during cold acclimation. The x-axis represents time during cold acclimation (d) and y-axis represents relative gene expression (arbitrary units)