Fig. 9

Molecular and phenotypic analyses of AIP1^KD lines. a Schematic representation of AIP1^KD line (GABI_645B06) indicating the T-DNA insertion in the third intron. b Relative mRNA levels of AIP1 in 21 day-old WT and AIP1 ^KD homozygote plants were determined by qRT-PCR. Data were normalized with AtUBI14 as reference gene. Data shown represent mean values obtained from independent amplification reactions (n = 3) and biological replicates (n = 2). Each biological replicate was performed with material collected from a pool of at least six plants. Bars indicate mean ± standard error of biological replicates. A statistical analysis was performed by t-test (p-value <0.05). Asterisks (*) indicate significant changes between samples. c Comparative analyses of the number of reproductive structures in 30 day-old AIP1 ^KD and WT plants. The graphs show the number of inflorescences containing young flowers per plant (left), inflorescences containing developed flowers per plant (middle), and inflorescences containing visible siliques per plant (right). Data has been quantified in ten plants. Values shown are means derived from two independent experiments. Bars indicate mean ± standard error of biological replicates. A statistical analysis was performed by t-test (p-value <0.05). Asterisks (*) indicate significant changes between control (wild-type) and samples. d (Left panel) Comparative analyses of developmental timing of flowers in 30 day-old AIP1 ^KD and WT plants. Values shown are means derived from two independent experiments. Bars indicate mean ± standard error of biological replicates. A statistical analysis was performed by t-test (p-value was 0.06). (Right panel) Photographs of dissected representative inflorescences of WT and AIP1^KD plants