Fig. 6

Transient expression of the PpNAC3 promoter fused to GFP in N. benthamiana leaves. a Diagram of constructs used for the Agrobacterium-mediated transient expression assay in N. benthamiana leaves. b Plants of N. benthamiana used for agroinfiltration. The constructs were agroinfiltrated as shown in the image. We used different leaves of the same plant for each construct, and the experiment was performed in triplicate. After 48 h, the leaves were treated with 100 μM MeJA or with water as a control. c Total RNA was isolated from N. benthamiana leaves and GFP or PR4 expression was studied at 2, 8 and 24 h after MeJA or water treatment using qPCR. Data were normalized to NbActin as a reference gene. Expression levels are relative to the values at time zero. The mean values ± SE are indicated. Asterisks indicate significant differences between the two treatments