Fig. 3

Expression patterns of PpNAC2 and PpNAC3. a Representative images of the pine seedlings with cotyledons 0.5, 1.0 and 2.0 cm in length and 2-months-old plantlets used in this study. b qPCR analysis of PpNAC2 and PpNAC3 transcripts in different organs of the P. pinaster seedlings and plantlets. Total RNA was extracted from different samples and reverse transcribed. The cDNA was amplified using specific primers for each gene, as described in Additional file 4: Table S1. The expression data were normalized using a geometric mean of the reference genes (ACT, 40S and EF1α). Analysis was performed three times on three independent biological samples, and the mean values ± SE are indicated. (C) Cotyledons, (N) Needles, (H) Hypocotyl, (S) Stem and (R) Root