Fig. 2From: Changes in hormone flux and signaling in white spruce (Picea glauca) seeds during the transition from dormancy to germination in response to temperature cuesChanges in ABA, ABA metabolites, and ABA signalling components during the transition from dormancy to germination of white spruce seeds. a Profiles of ABA and its metabolites as determined by UPLC/ESI-MS/MS during moist-chilling at 3 °C (0, 10, and 21 days) and during germination (6, 24 and 80 h) and seedling growth (9 d). Each data point is the average of two biological replicates. b Schematic of ABA biosynthesis, signalling, and catabolism. c Transcript levels of ABA metabolism and signalling genes and selected downstream targets during moist-chilling (0, 10, 21 d), germination (6, 24, 80 h) and seedling growth (9 d) (black bars). Also shown are previously chilled seeds placed in two control treatments - 6 h germination conditions under darkness at 30 °C (dark grey bar) or 20 °C (light grey bar). Each data point is the average of three biological replicates. Bars indicate the SEM. Note: two superscript asterisks (**) indicate that the gene is only annotated in angiosperms; one asterisk (*) indicates that the gene has been annotated in gymnosperms but not in white spruce; no asterisk indicates that the gene has been annotated in white spruceBack to article page