Fig. 6

Effects of proline on SHY2 loss- and gain-of-function mutants. a-f Longitudinal sections of roots, at 7 dag, from wild-type (a), p5cs1 p5cs2/P5CS2 (b), shy2-31 (c), p5cs1 p5cs2/P5CS2, shy2-31 (d), shy2-2 (e), shy2-2 + proline (f), showing that in p5cs1 p5cs2/P5CS2, shy2-31 the root meristem is as large as the meristem of shy2-31, and in proline-treated shy2-2 larger than in untreated control. Black arrowheads indicate the QC (bottom arrowhead) and the TZ (top arrowhead). Bars = 20 μm (a-f). g The number of root meristem cells of the mutant lines shown above, from a to f, are reported as graphic bars. Error bars indicate Standard Error (SE). Student’s t test for wild type v/s mutant lines p*** < 0.001; Student’s t test for shy2-2 v/s shy2-2 + Pro p*** < 0.001