Fig. 5

CsaMLO8 transcription is induced after inoculation with Podosphaera xanthii in hypocotyl tissue, but not in cotyledon or true leaf tissue. Susceptible (a) and resistant (b) cucumber seedlings were inoculated with a P. xanthii spore suspension. Prior to and 4, 6, 8 and 24 h post inoculation, hypocotyl, cotyledon and true leaf tissue were harvested and immediately frozen in liquid nitrogen. Relative quantification of CsaMLO8 expression was performed by qRT-PCR. Fold changes were normalised relative to CsaMLO8 expression prior to inoculation. Bars represent the average fold change over three independent biological replicates. Error bars indicate standard errors of the mean. Asterisks indicate significant differences to the expression prior to inoculation (Student’s T test, P < 0.05)