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Fig. 9 | BMC Plant Biology

Fig. 9

From: Oligogalacturonic acids promote tomato fruit ripening through the regulation of 1-aminocyclopropane-1-carboxylic acid synthesis at the transcriptional and post-translational levels

Fig. 9

Gene expression and protein variation of LeWAKL2. a Gene expression levels of LeWAKL2 by 2 h after OGs treatment, and the values were presented relative to the Actin. The experiment was repeated in triplicate, and the asterisks indicate statistically significant differences compared with the control group (*P < 0.05; **P < 0.01, Student’s t-test). b Protein accumulation of LeWAKL2. Total 25 μg protein were loaded on each line to separate through 8 % SDS-PAGE gel, and anti-LeWAKL2 was incubated with membrane overnight at 4 °C. The relative intensities of LeWAKL2 were quantified with Adobe Photoshop CC for OGs treated group. We used the first hour LeWAKL2 protein band intensity as standard since no signals were detected for the control group. Ponceaux dyeing was used to verify the protein amount. The experiment was repeated in triplicate. A representative gel is shown. Significant differences of four groups at each time point were indicated by different letters (Tukey’s HSD, P < 0.05)

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BMC Plant Biology

ISSN: 1471-2229

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