Fig. 5
Similar responses of ArcLight and CBL1-SEpHluorinA227D to eATP. The traces derived from the regions of the root indicated by the connecting lines (MiCAM image at 0 s, 20x objective) are displayed over a time period of 1092 s. Either 2 mM ATP or buffer was added at approximately 100 s as indicated by the blue arrows. Fractional fluorescence changes (%dF/Fmax) were calculated by the BV-Analyzer software supplied with the MiCAM camera. The divisions of the Y-axis are set at 6 %. The X-axis shows time in seconds. Top: Responses of ArcLight to eATP addition are shown for multiple cells within the root. All cells show a qualitatively similar response. The background trace, which remains unchanged following addition of eATP, is shown above the MiCAM image. Bottom: Response of CBL1-SEpHluorinA227D to addition of eATP or buffer. The observed trace resembles that seen with ArcLight. The background trace is shown in black