Messenger RNA exchange between scions and rootstocks in grafted grapevines

BMC Plant Biology

Table 1 Graft genotypes and combinations, growing conditions, tissue sampling, and numbers of genes with mobile mRNA reads detected

Growing condition	Sampling time	Genotype		Scion			Rootstock			Total no. of genes	Average transmission rate
Growing condition	Sampling time	Scion	Rootstock	Tissue sampled	Mapped Reads^a	No. genes	Tissue sampled	Mapped Reads	No. genes	Total no. of genes	Average transmission rate
in vitro ^b	4 weeks after grafting	V. girdiana	V. palmata	shoot, leaf, stem	98.4 M	1130	shoot, leaf, stem, root	112.3 M	646	2679	0.0238
in vitro	4 weeks after grafting	V. palmata	V. girdiana	shoot, leaf, stem	104.9 M	1125	shoot, leaf, stem, root	100.5 M	747	2679	0.0238
Field^c, pH5.5^d	11 years after grafting	V. vinifera cv. ‘Riesling’	Vitis hybrid ‘C3309’	young shoot	64.8 M	80	small root	79.7 M	555	987	0.042
Field, pH6.5^e	11 years after grafting	V. vinifera cv. ‘Riesling’	Vitis hybrid ‘C3309’	young shoot	91.6 M	134	small root	66.4 M	517	987	0.042

^aThe number of 101-bp RNA-Seq reads (in millions) mapped to the grape reference genome
^bEach in vitro graft combination had three or more grafted plants which were bulked in tissue sampling
^cThe grafted plants were planted in the field in 2003. Tissues from six plants from each field condition were pooled as a bulk sample
^dSoil was untreated
^eSoil was treated with limestone to improve the soil pH level

ISSN: 1471-2229