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Table 2 Summary of PCR analysis for T0 transgenic plantsa

From: Development of marker-free transgenic Jatropha curcas producing curcin-deficient seeds through endosperm-specific RNAi-mediated gene silencing

Name

Gus linker

F1-R2

Hpt

F1-R1

F2-R2

MD44

T0-1

+

+

+

+

T0-20A

+

+

+

+

+

T0-25A

+

+

T0-29

+

+

T0-30

+

T0-33

+

+

+

+

+

T0-34

+

T0-35

+

+

+

+

+

T0-36

+

+

+

T0-40A

+

+

+

T0-40B

+

+

T0-48

+

+

+

+

+

  1. aDNA primer pairs for PCR amplification are as follows: Gus linker, Gus-L-F and Gus-L-R; F1-R2, F1 and R2; Hpt, Hpt-F1 and Hpt-R1; F1-R1, F1 and R1; F2-R2, F2 and R2. The DNA sequences of the primers are listed in Table 1