Fig. 5

Western blot analysis of curcin proteins in the endosperm of transgenic T2 seeds of L1 and L35. a Western blot analysis with total proteins isolated from the endosperm of T2 seeds of L1. T0-1/T1-1/T2-2 and T0-1/T1-1/T2-9 are T2 individuals belonged to transgenic line L1. b Western blot analysis with total proteins isolated from the endosperm of T2 seeds of L35. T0-35/T1-1/T2-1 and T0-35/T1-1/T2-2 are T2 individuals belonged to transgenic line L35. Proteins isolated from the endosperm of mature jatropha seeds were separated by SDS-PAGE and curcin proteins were detected by anti-C1 antibodies. Proteins stained with Coomassie brilliant blue in duplicate SDS-PAGE gels serve as protein loading controls. The arrows indicate the positions of C1 in western blot analysis and SDS-PAGE gels, respectively