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Fig. 4 | BMC Plant Biology

Fig. 4

From: Development of marker-free transgenic Jatropha curcas producing curcin-deficient seeds through endosperm-specific RNAi-mediated gene silencing

Fig. 4

Southern blot analysis of transgenic plants. a to c Southern blot analysis of MD44 and T2 plants derived from transgenic T1 plant T0-1/T1-1. d to (f) Southern blot analysis of MD44 and T2 plants derived from transgenic T1 plant T0-35/T1-1. Plant genomic DNA was digested by single restriction enzymes HindIII (H) or XbaI (X) (a, b, d and e), or with the combination of PmlI and PmeI (c and f) and then fractionated on a 0.8 % agarose gel. Southern blots were probed with T Nos (a, c, d and f) or Hpt (b and e) probes. M, DNA molecular marker; Kb, kilobase

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