Fig. 1

A schematic diagram of the T-DNA region of the construct pCMFC1 and Cre/loxP-mediated DNA recombination (Map not drawn to scale). Region flanked by the two loxP sites (filled boxes) in the upper diagram is the loxP fragment, which is excised by Cre/loxP-mediated DNA recombination after β-estradiol induction [26]. LB and RB in pCMFC1 are drawn with open boxes, whereas the broken LB and RB due to T-DNA integration into plant genome are shown with hatched boxes. T _Nos , terminator of nopaline synthesis (Nos) gene; CRE-int, bacteriophage P1 Cre recombinase gene with an intron; O _LexA-46 -P _35Smini , eight copies of LexA DNA binding site fused to the −46 CaMV 35S mini-promoter; Hpt, coding region of hygromycin phosphotransferase gene; P _Nos , Nos gene promoter; T _E9 , rbcS E9 terminator; XVE, open reading frame encoding chimeric transactivator containing the regulator domain of an estrogen receptor; P _35S , CaMV 35S promoter; F1, R1, F2 and R2, DNA primers used for PCR analysis to detect Cre/loxP-mediated DNA recombination (Table 1). Only one PmlI or PmeI cleavage site is identified in the T-DNA region. Two HindIII or XbaI cleavage sites are indicated in the map, respectively. Other HindIII or XbaI sites in the regions flanked by the two HindIII or XbaI sites are not shown. DNA probes for the Nos terminator (T _Nos ) or the coding region of the Hpt gene (Hpt) are indicated