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Fig. 7 | BMC Plant Biology

Fig. 7

From: Genetics and physiology of cell wall polysaccharides in the model C4 grass, Setaria viridis spp

Fig. 7

Micrographs of stem transverse sections. a, c Bright-field light micrographs of a toluidine blue-stained survey section. b, d Fluorescence light micrographs using an antibody conjugated to Alexafluor 488 where (1,3;1,4)-β-Glucan is indicated by green fluorescence. e Fluorescence light micrograph with a secondary antibody only as negative control. f TEM micrograph of walls of ground tissue cell. E epidermis; BS bundle sheath; CcM Chloroplast-containing mesophyll; SF, sclerenchyma fibre; SC, sclerenchyma cylinder, GT, ground tissue; CyGT, cytoplasm of ground tissue cell; VBW, vascular bundle wall. Arrows indicate gold labelling. Magnifications: a, b, e 100 x; c, d 400 x. Scale bars: a, b, e 100 μm; c-d 50 μm; f 1 μm. Exposure time: b, d, e 2.6 s

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