Fig. 2From: Low temperature-induced DNA hypermethylation attenuates expression of RhAG, an AGAMOUS homolog, and increases petal number in rose (Rosa hybrida)Expression pattern of RhAG in response to low temperatures during flower development. Expression of RhAG was monitored by quantitative RT-PCR. Two-year-old rose plants were cultivated at 25/15 °C (black column) or 15/5 °C (white column). Flowers were collected at stage 1 to stage 4. RhTCTP was used as an internal control for all the tested genes. The expression level of RhAG at stage 1 grown at 25/15 °C was defined as 1.0. Values are means ± SD (n = 3). Asterisks indicate significant differences calculated using the t test (**p < 0.01; *p < 0.05)Back to article page