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Fig. 8 | BMC Plant Biology

Fig. 8

From: Two homolog wheat Glycogen Synthase Kinase 3/SHAGGY - like kinases are involved in brassinosteroid signaling

Fig. 8

Response of wheat seedlings to epiBL, Bikinin and PCZ foliar spray. Selected concentrations of epiBL, Bikinin and PCZ were sprayed 4 times on the leaves of wheat seedlings grown for 14 days on vermiculite in an incubator under the same conditions as the in vitro grown seedling. Values in (a) represent mean values and standard deviations of seedling aerial part length measured after 14 days of culture. Values in (b), (c) and (d) represent the percentage of seedlings that had a given inclination angle (angle between the first leaf blade and sheath) upon epiBL (b), PCZ (c) and Bikinin (d) treatment after 14 days of culture. e shows representative inclination angles upon epiBL treatment. In independent experiments, 27–30 seedlings were monitored for each epiBL concentration and for the control. Thirty, 15, 30, 40, 43, 44, 41, 44 seedlings were recorded for respectively 0.3% DMSO, 10 and 30 μM Bikinin, 0.25 % DMSO, 40 and 50 μM Bikinin, 0.4 % DMSO, 80 μM Bikinin. Finally, 12–13 seedlings were measured for the control and each PCZ concentration. * T.test p <0.05 (p = 0.01026141 for 10 μM epiBL), ** T.test p <0.01 (p = 0.00787367 for 10 μM Bikinin).***T.test p <0.001 (p = 3.5079E-07 for 5 μM PCZ, p = 3.75455E-09 for 30 μM PCZ)

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