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Fig. 5 | BMC Plant Biology

Fig. 5

From: Two homolog wheat Glycogen Synthase Kinase 3/SHAGGY - like kinases are involved in brassinosteroid signaling

Fig. 5

Effect of Bikinin on BR target gene expression levels in TaSK1.2-1 severe dwarf lines. mRNA levels of TaSK1 (a), BR-biosynthetic CPD (b), BR-upregulated SAUR-AC1 (c) and BR inactivation BAS1 (d) genes were quantified by qRT-PCR in TaSK1-A.2-1 severe dwarf (SD) lines either treated with 30 μM Bikinin or grown on control medium for 8 days. mRNA levels of BR target genes were in parallel quantified in Columbia seedlings either treated with 30 μM Bikinin or grown on 0.3 % DMSO for 7 days. All results are means +/- standard deviations. Individual reactions were done in triplicate (technical replicates). Expression levels were normalized to those of Polyubiquitin10. Similar results were obtained when normalizing expression levels to those of EF-1alpha (Additional file 2)

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BMC Plant Biology

ISSN: 1471-2229

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