Skip to main content
Fig. 2 | BMC Plant Biology

Fig. 2

From: Two homolog wheat Glycogen Synthase Kinase 3/SHAGGY - like kinases are involved in brassinosteroid signaling

Fig. 2

BR target gene expression levels in TaSKs.2-1 transgenic lines. mRNA level of BR-biosynthetic CPD, BR-upregulated SAUR-AC1 and BR inactivation BAS1 genes were quantified by qRT-PCR in TaSK1.2-1 (a-h) and in TaSK2.2-1 (i-l) lines. Relative expression levels in representative lines having same size and morphology as wild type plants (approx. 40 cm), in representative semi-dwarfs and in representative severe dwarfs (with and without flowers, referred as dwarf) were compared to expression levels in Columbia plants. In the recorded samples, 36 out of 180 TaSK1.2-1 transgenic plants were severe dwarfs and 69 had a normal size. Eleven out of 42 TaSK2.2-1 transgenic plants were severe dwarfs and 18 had a normal size. All results are means +/- standard deviations. Individual reactions were done in triplicate (technical replicates). Expression levels were normalized to those of Polyubiquitin10. Similar results were obtained when normalizing expression levels to those of EF-1alpha (Additional file 1)

Back to article page