Fig. 4

Impact of rising EGTA concentrations on adenine nucleotide transport of full-length and N-terminally truncated AtAPC2. Transport via recombinant AtAPC2 (a) and via the mutated version lacking its N-terminal domain (b). Import of [α³²P]-ATP into P_i loaded proteoliposomes (black squares) and of [α³²P]-ADP into ATP loaded vesicles (gray circles) was allowed for 5 min. Transport without EGTA was set to 100 % and transport in presence of rising concentrations of externally added EGTA (0 - 500 μM) was calculated accordingly. Data represent net values of ATP/P_i and ADP/ATP import minus the respective control (non-loaded vesicles) of three independent replicates. Standard errors are given