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Fig. 2 | BMC Plant Biology

Fig. 2

From: In vitro analyses of mitochondrial ATP/phosphate carriers from Arabidopsis thaliana revealed unexpected Ca2+-effects

Fig. 2

Ca2+-impact on ATP transport via AtAPC1-3. Effect of rising Ca2+-concentrations (0–500 μM) on transport mediated by recombinant AtAPC1 (a, b), AtAPC2 (c, d) and AtAPC3 (e, f). Transport of 50 μM [α32P]-ATP was conducted in absence (black rhombs) and presence of supplemental MgCl2 (gray rhombs). Transport was allowed for 5 min and is given in nmol mg protein−1 h−1. Ca2+-dependent stimulation of ATP/Pi hetero-exchanges (a, c, e) and ATP/ATP homo-exchanges (b, d, f). Non-loaded liposomes (non-filled rhombs; negative control) showed only marginal accumulation of ATP and the corresponding rates were unaffected by MgCl2 addition. Data represent mean values of three independent replicates, standard errors are given

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