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Table 1 Transcript accumulation and activity of cell wall invertase, and sugar content in stem bases of cuttings of wild type, pHell and PhAOC-RNAi plants

From: Jasmonates act positively in adventitious root formation in petunia cuttings

parameter

unit

WT

pHell

PhAOC-RNAi

   

line 4

line 10

line 4

line 10

line 15

3CL9414-transcripta

2−∆Ct

20.9 ± 10.1

19.4 ± 7.5

23.1 ± 3.0

19.2 ± 4.6

14.6 ± 7.2

17.8 ± 5.4

invertase activityb

pkat/mg protein

12.6 ± 3.7

14.1 ± 3.3

10.2 ± 5.2

10.1 ± 4.2

9.7 ± 3.9

8.3 ± 4.4

glucosec

μmol/g f.w.

0.2 ± 0.1

0.2 ± 0.0

0.2 ± 0.1

0.2 ± 0.1

0.2 ± 0.1

0.1 ± 0.1

fructosec

μmol/g f.w.

0.4 ± 0.3

0.2 ± 0.1

0.1 ± 0.0

0.5 ± 0.4

0.4 ± 0.4

0.5 ± 0.3

sucrosec

μmol/g f.w.

1.8 ± 0.6

1.6 ± 0.2

1.1 ± 0.6

2.7 ± 0.9

2.3 ± 0.7

2.4 ± 1.2

  1. All data are given as mean values ± SD from eight independent, biological replicates. Data were compared by one-way-ANOVA and did not show significantly different values according to Tukey’s HSD test
  2. aTranscript data are relative to PhRSP13 determined at 4 hpe
  3. bCell wall invertase activity determined at 6 hpe
  4. cSugar levels determined at 192 hpe