Fig. 1
From: Jasmonates act positively in adventitious root formation in petunia cuttings
Accumulation of PhAOC transcripts, AOC protein and activity in stem bases of cuttings of Petunia hybrida expressing PhAOC-RNAi. (a) Stem bases were collected from leafy cuttings of wild type (WT, green), two lines expressing the empty vector (pHell, blue) and three lines expressing a PhAOC-RNAi construct (red) at one hour after excision. Relative PhAOC transcript accumulation shown as 2−ΔCt values in relation to the reference gene PhRSP13 was determined by qRT-PCR. (b) AOC protein was detected immunocytologically in cross sections of stem bases using an antibody specific binding to AOC. Note the occurrence of AOC in plastids visible as distinct dots within cells (inset in WT). Bars represent 100 μm and 25 μm in overviews and inset, respectively. (c) AOC enzyme activity is given as nmol g−1 FW of enzymatically formed OPDA. Each value is represented by the mean of eight independent biological replicates ± SE. Different letters within each graph designate statistically different values (one-way-ANOVA with Tukey’s HSD test, P < 0.05)